【文獻標題】Hydroxyproline supplementation on the performances of high plant protein source based diets in turbot (Scophthalmus maximus L.)

【作者】Yunzheng Liu，Gen He，Qingchao Wang，et.al

【作者單位】中海洋大學（Ocean University of China）

【文獻中引用產品】

試劑L-羥脯氨酸

【關鍵詞】Turbot，Hydroxyproline，Plant protein，Muscle texture

【DOI】http://dx.doi.org/10.1016/j.aquaculture.2014.07.002

【影響因子(IF)】4.70

【出版期刊】《Aquaculture》

【產品原文引用】

L-hydroxyproline (N99% pure) was obtained from Hengyuan Biotech (Shanghai, China) Co., LTD. Fishmeal, soybean meal, corn gluten meal,wheat gluten meal, peanut meal, ａnd beer yeast were used as the primary protein sources. Fish oil ａnd soybean lecithin were used as the lipid sources. Wheat flour was used as the carbohydrate sources. Seven isonitrogenous ａnd isoenergetic diets were formulated: a reference diet (FM) containing 60% fish meal ａnd three other diets (40I, 50I ａnd 60I) in which 40%, 50% ａnd 60% fish meal was substituted. Based on the supplementation levels used in reports (Asknes et al., 2008; Zhanget al., 2013), experimental diets with plant protein substitutions were either supplemented with 0.6% hyp or not. All ingredients were grounded into fine powder through 178 μm mesh. Ingredients were blended with oil ａnd water then added. Pellets (3 mm × 4 mm) were made automatically by pellet-making machine ａnd dried for 12 h in a ventilated oven at 40 °C. The hyp concentrations after diet preparation were quantitated as 0.23% (FM), 0.16%(40I), 0.58%(40I HYP), 0.18% (50I), 0.62% (50I HYP), 0.14% (60I) ａnd 0.57% (60I HYP). DL-Methionine, L-Threonine,L-Histidine, ａnd Lys-H2SO4 (Crystalline amino acids) were supplemented to meet the essential amino acid (EAA) requirements of juvenile turbot based on the amino composition of FM diet. All the compositions of experiment diets were shown in Table 1.

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