【文獻標題】Chronic exposure to low environmental concentrations ａnd legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia ａnd provoke differential human health risk

【作者】Samwel M. Limbu，Li Zhou，Sheng-Xiang Sun，et.al

【作者單位】華東師范大學（East China Normal University）

【文獻中引用產品】

魚丙二醛（MDA）ELISA試劑盒

【關鍵詞】Low environmental antibiotics，Legal aquaculture antibiotics，Toxicological effects，Human health risk

【DOI】https://doi.org/10.1016/j.envint.2018.03.034

【影響因子(IF)】8.58

【出版期刊】《Environment International》

【產品原文引用】

After weight measurements ａnd counting, eight fish from each tank were sampled, individually anesthetized with MS-222 (20 mg/L) (tricaine methanesulfonate, Western Chemicals, Inc., Ferndale,Washington) ａnd sacrificed for tissue samples collection for biochemical assays, nutrients body composition ａnd digestibility measurements.Intestines, livers ａnd skins from O. niloticus were excised ａnd subsequently homogenized in a glass beds with 9 volumes (v/w) of 0.8% physiological saline. The homogenate was centrifuged (2500 rpm, at 4 °C for 10 min), the resulting supernatant was collected ａnd subsequently frozen at −80 °C for biochemical assays according to manufacturers＇ instructions. One whole intestine, liver ａnd a piece of skin from each fish were used in all analyses. On the days of analyses,malondialdehyde (MDA) concentration was determined by using enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai).Protease, lipase, amylase, lactate dehydrogenase (LDH), glutathione Stransferase (GST), superoxide dismutase (SOD), total antioxidant capacity (TAC), acid phosphatase (ACP), ａnd alkaline phosphatase (AKP)were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China), with their results recorded on a microplate reader (Epoch, BioTek, USA). The remaining fish (with viscera removed) were used for body composition ａnd apparent nutrients digestibility measurements according to standard methods (AOAC, 1995).

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原創作者：上海恒遠生物技術發展有限公司