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【文獻標題】Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto’s thyroiditis ａnd Systemic lupus erythematosus
【作者】BING TAN，WEIXIN YUAN，JINYING LI，et.al
【作者單位】浙江大學（Zhejiang University）
【文獻中引用產品】
【關鍵詞】cell therapy, experimental autoimmune thyroiditis, human amniotic epithelial cells, Hashimoto’s thyroiditis，immunomodulatory, Systemic lupus erythematosus
【DOI】https://doi.org/10.1016/j.jcyt.2018.04.001
【影響因子(IF)】3.84
【出版期刊】《International Society》
【產品原文引用】
Serological assays
Sera cytokine levels of IL-17A, IFN-γ, IL-4, IL-10 and TGF-β of EAT ａnd SLE mice were measured by ELISA. All ELISA kits were from Hengyuan Biological Technologies (Shanghai, China). Specific experimental steps were performed according to the manufacturer’s instructions.
Cytokine production in vitro
Splenocytes of EAT mice at the peak of disease (35 day) were stimulated with pTg in the presence or absence of hAECs. Cell-free culture supernatants were collected after 48 h by centrifugation. Cytokine levels of IL-17A, IFN-γ,IL-4, IL-10 and TGF-β were measured by ELISA(Hengyuan BiologicalTechnologies, Shanghai, China).
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